◆ SpookStack

'?? V* s r - - * •••^r "* Request fornrenewal of support for theproject j Investigation of the~seed of Rlvea Coryabeaa toloiuq Summary of work, November 1, 1955 -September 25, 195G* Physical examination of the seed used in this work reveals that two "typos" have been under, investigation# . Taxonomic ally all the seed have been called Rlvea Corymbosa although one variety (the seed are the larger 1 of the two. . varieties) originated in Mexico and one variety originated in Cuba* The literature..on the subject of ololluqjii, as far as I can ascertain indicates that its use as a psychic drug has been restricted to Mexico# Thl^^dnpa alone would indicate the probabili ty that the CUbo?^>oT^fce^y lnck3 some physiologically active comporieht/^ich tK^\Mexican variety contains# Experimental work /in this laboratory has been largely restricted to the C^^ah variety | (small _ seed ainco only a vary small amount of the Mexican variety ha a been available * The chemical work, altfacjzffi. not desired to compare the conpp^vio^xQf varle ties, has indicated some differoncor in thbOtwo, Also physiological tests on human a are Indicative of a difference in the two—j • .. varie ties • Thla work is E&jh too ihcbhclusivo to be nuch — more than s peculation at tAin time*// In addition to thb^iaolb^on^f^glucose and hydro- quinone as const! tuent^oj\ololluqui as reported la the last progress report,/^oNrystall ine fractions have been . isolated which seem i^dtherNfe be alkaloid or glycoside. Due to the small quantity ofOeeed available tho amount of these crystalliifM froo^rlens is extremely small and . • little more than q^Viits^ve tests have been made on then# Experimes^l teohni^ba-j^espeoially in extraction and separationvhave been perfected which will be uaedto isolate uu nett t o of tho drug which, just today, has become a r illable In sufficient quantity to make the investigatio n profitable* / Plans for fu ijre investigations Kovr that 50 pounds of the Cuban vorloty and 10 pounds of the -rexican variety ere available, the following lines of inveatigatiea will be aotiyely^l^sued# ' 1* Mtermine conclusively if there is a chemical difference in the tsfe varieties of seed# 2* Obtain as many impure": components from each variety, using previously perfected techniques, aa is possible# 3, Submit for physiological testing each "pure" component# _ 4* Subject each "pure" component to chemical examination to determine tho typo of compound, whether it la a known compound and its chemical composition if it is v new compound#