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-v^r;c^r:oTiCE 3 . ' * -c X SENSITIVE INTELLIGENCE RESEARCH PROPOSAL DURCES AND METHODS INVOLVED 1359 - 1960 The research to be undertaken during the next 12 months for which a 8 tt financial support is hereby requested will be devoted to the analysis of 7 the hypothalamic and endocrine mechanism of^ stress and chemical agents.** that modify it. The investigations proposed are a direct continuation and • outgrowth of those .supported in the previous year. The screening procedures to be used have been markedly widened — in scope to embrace a consideration of botb the metabolic and enzymatic effects of stress or of the chemical agents studied. ~~ ~ A novel screening procedure which depends upon the survival time of animals maintained without food or water has been devised and has already been put to practical use in demonstrating the role of the hypothalamus in survival. In addition to this over-all survival j; screening technic , screening technics have been devised for inves^igat- lug separately the role of water and fat metabolism Jh stress. - It is proposed to continue work on the negative phase of stress - which we have previously delineated by. study of the egg white response in rats. (See research report for 1957 - 19 5 8}- ~ * As an outgrowth of experiments performed in 1958 it is now possible to evaluate therapeutic agents at the enzymatic level. See 7 research report enclosed. This technic of using a battery of enzyme systems in vitro^as a method of evaluating the biological effectiveness of newly systhesized organic compounds opens up a whole new line of investigation and is the most rapid method of evaluation that has been devised to date and p^pbably the most basic. It is proposed that we exploit this new approach to our utmost in the next twelve months. *a r i