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(Rev. 01-31-2003) FEDERAL BUREAU OF INVESTIGATION Precedence: ROUTINE Date: 11/16/2004 ALL INFORMATION CONTAINED To: Washington Field HEREIN IS UNCLASSIFIED oe f DATE 12-17-2008 BY 60924 UC BAN/DE/TH From: Washington Field . AMX 1 Contact: Approved By: — Case ID #: 279A-WF~222936-USAMRIID (Pending) —- 105 2 _ Title: AMERITHRAX; _ woe one nme MAFOR “CASE: LB G0 nr neces Seaemaninrs canna TN ara 9a Synopsis: To provide a summary of the investigative efforts into the water purification systems in USAMRIID and resulting water collection. Details: In 2004, the Amerithrax investigation looked into the U.S. Army Research Institute of Infectious Diseases' (USAMRIID's) water sources and the filtration systems used by both Buildings 1425 and 1412. Interviews of Principle Investigators, technicians, and Facilities Management personnel were conducted to determine the different purification methods in each building, and the types of water used for Bacillus anthracis (B.a.) studies. On 2/25/2004, stated that the water used_to make media and reagents in the Suites of Building ame from a large filtration system in suite This - system was later confirmed to be a Millipore filtration be system. For washing and storage of B.a., utilized pyc bottles of sterile water for injection purchased from B. Braun (product number S$9200-SS). sf iis in the process of obtaining the same bo es Of Sterile water through CBSU. Bruce Ivins learned from that distilled deionized Milli-Q water is used for his Ames spore preparations. stated in a 3/2/2004 interview that a deionized water filtration system is housed in the penthouse of Building [____] This deionized water is further purified through a Millipore system prior to use. All the ‘laboratories in Building have access to this water