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To: ‘Washington Field @...:: Washington Field @ Re: 279A-WF-222936, 01/11/2006 11, 1983 and was entitleal One page described the isolation of Ba strain Vollum-1 in January 1948 in Onderstepoort, South Africa. Another Ba strain, G28, was isolated in 1939 in Pe South Africa. bic Notebook was issued November 18, 1983 to and was entitled describes obtaining Ba toxins from R-medial cultures from Vv ins on April 2, 1984. Several toxin strains, including Ames, were obtained by[ A March 27, 1984 entry describes an aerosol challenge using MDPH and Sterne spore-immunized guinea pigs. A similar experiment was conducted between February 28 and April 10, 1984, and on June 20, 1984. Noteboo was issued March 17, 1993 yo[ id and was entitled vins made an entry into this notebook on Augus ; , in which he described combining polyclonal antisera, along with sera from guinea pigs immunized with Sterne and then challenged with Ames, in an effort to protect the guinea pigs from the challenge. NotebookL__] was issued September 30, 1 [and was entitled Lo received six fermenter samples from information about the samples was provide NotebookL___] and was entitled ut no other was issued September 29, 1995 to On April 4, 1995, Notebook and was entitled used RMR 1029 Ames spores in B 1 provided in the form of a table. The experiment was a rabbit immunogenicity study seeking the effects of alhydrogel. No was issued April 4, 1985 ear was ther obscured] to and was entitled Ce obtained Ames and other Ba strains from Ivins on June 8, 1987 to = prepare stock plates in [_|then ran the samples through a — gel electrophoresis. obtained more Ames samples from Ivins — at a later date to test them against different PCR primers. also streaked 5% sheep blood plates using four samples from the bacterial stock cultures. One of these samples was BAOO76, or a- Ames-1. The experiment details have been abbreviated here, but the full experiment was copied by writer and is available for review. bYF