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fa FD-302a (Rev. 10-6-95) @ a. @ 279A-WF-222936-USAMRIID 10° or 10°° spores/mL. During a challenge, spores were deposited in water in the AGI. The solution remaining in the AGI after the challenge was approximately 6-10 mL in volume. The AGI solution was given to IVINS, who conducted the post-challenge plating. About 1/10 mL was needed for post-challenge plating, and the remaining volume from the AGI could have stayed in room 115 for weeks afterward, though it usually did not stay there that long. The only samples of Ba remaining after a challenge were contained in the AGI and the plates that were created post-challenge. Used plates were discarded in trash bags, which were taken by the caretakers when they became full. .. Animals for pox challenges were kept in BuildingL___]| did not recall any Ba being stored there. did not recall any Ba being stored in hallways of uildin zoon{__| was There shou there that and he thought there was probably no Ba in room[ | laboratory which was used for pox. een Ba in suite the only agents used recalled were The walk-in cooler on the first floor of Building was an unlocked, generally common cold room for people without a refrigerator in their laboratories;[__ ‘aia not recall if Ba was stored there. The upstairs walk-in cooler, room[___] was often down and moldy, and was not used a lot. If any Ba was stored there, it probably belonged to Fd did not recall any specific instances when Ba might have been stored there. did not know if Ba was stored in Building 1425, rooms , or in suite thought there should not have been Ba in suite and did not recall seeing any there. did perform Ba_sprays in suiteL__] Ba sprays occurred in while Building, ~ as down for thought the renovations of Building] | in renovations. were probably completed by the end of 1997. Ba was not store , any hallways of Building 1425. Inspector[___|showea[ cour copies of of a flask labeled "OCT 97 GLP Ames Spores." did not recall ever seeing the flask or anything like it. The Ba that was stored in the challenge areas were generally not in flasks. Samples brought by IVINS were usually contained in 50 mL conical tubes that were taped on top. Samples from the AGI were kept in 15 mL tubes. Copies of the photographs are maintained in a 1A envelope. conimusinorepanzor [on af /2006 ste 2 be b? 7 Ag faa G F