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FD-302a (Rev. 10-6-95) 279A-WF-222936 Continuation of FD-302 of rr ee , oF /03/06 50 ,» Page After an aerosol challenge, all material from the challenge was autoclaved. The Ba Ames left in the all-glass impinger (AGI) was not autoclaved right away. [_] provided the following description of an aerosol challenge: Based_on the number off animals being used in an aerosol challenge,[__] would calculatd the number of spores that[ _] would need for the challenge. BRUCE IVINS would send over the required amount of spores in an Erlenmeyér flask or a 50 mL conical tube. IVINS would provide[ __] with he Ba Ames from Dugway. IvinsC__] did not complété any paperwork documenting the internal Transfer of Ba until approximately mid-2002. IVINS began using an internal transfer sheet and USAMRIID followed very soon after. provided Agents copies of the paperwork documenting the internal transfers of Ba from June 2002 until present. These documents were_placed in an FD-340 and submitted to the 1A section _of the file. The aerobiology group began conducting the plate counts approximately 3 years ago. The aerobiolo division stores AGI’s in a lock box in the cold storage room The lock box has only been in use since approximately June 2002. The material leftover in the AGI’s would usually be approximately 3 orders of magnitude less concentrated than the starting material. rors ~IN zal wb QQ