◆ SpookStack

FD-302a (Rev. 10-6-95) . oe ron) <4 OQ 279A-WP-222936 Continuation of FD-302 of | | ,On_ 01/03/06 , Page 7 Since_approximately 1998 there was an old lyophilizer located in room| USAMRIID has recently replaced the old lyophilze j new one. [ ___has never lyophilized Ba natezial. [ | believes that somebody using a lyophilizer to dry the Ba material in the anthrax letters would have created a big — people would have gotten sick, and left a definite footprint. believes that acetone drying is a lot easier, safer, and does not require specialized equipment. An aerosol challenge using dry Ba_would nev USAMRIID because of the political/fallout. pom never occur at [__Jwoula never store Ba in room technician wou autoclave AGI and plate material before leaving laboratory. If the autoclave i was already in use, FF would store material left to be autoclaved in the hood. Material waiting to be autoclaved could possibly be in the hood until the next day. Autoclaved material would be packaged and labeled appropriately. A piece of autoclave indicator tape was affixed to material to be autoclaved. Usually the agent to be autoclaved would be written on the outside in case somebody had an exposure to the material. Writing the agent on the outside allowed for a more timely and appropriate post-exposure treatment of person’s exposed. Hot material, if not refrigerated or frozen, could only be in the hood or in the autoclave. [ —pvelieves thawing frozen Ba is a “no, no”, since viability o @ sample is lost each time freeze-thawing occurs.