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” FD-302a (Rev. 10-6-95) 279A-WF-222936-LEADS Continuation of FD-302 of | | ,On 08/18/2005 _,Page = 4 » correlation of samples removed from the freezer to those destroyed/sterilized. Every autoclave session had a control spore strip present which would be used to make sure the autoclave cycle was completed. The chain of custodies, frozen logs, and autoclave logs were all handwritten in books, not computerized. Similar records were maintained at USAMRIID. [Jnotea that at{ Josarerr [enare were no genera ogs maintained which would show how much organism was grown from the samples, or records to reflect how much of the grown organism was used in testing, and then subsequently destroyed. Some reconstruction of this could be done by reviewing the laboratory notebooks of all of the researchers who worked with the suspect agents. when stock samples were removed from the freezer, they were isolated via streak plates and grown in broth. Then for long term storage, the organism was spun down, placed in a tube with glycerol, quick frozen in a dry ice/ethanol solution, then placed in a -70 freezer. [ never lyophilized the organisms. Lyophilization would only Be necessary if an organism was being stored for an extremely long time. Organisms were never distributed to researchers outside of the facility was responsible for the organisms, though eo eir origin, used_in Whereas| bwould have been given[ _]working organisms by at USAMRIID.