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To: Washington m From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 however, there were occasions when the room was shared with other PIs. For example, in 1998 and 1999 utilized room for Brucelia study work. It was documented during several interviews that Ivins' group did not keep room very clean and tidy. Post-challenge agar plates were left on counters, the incubators were left full of material, samples in the refrigerator were not disposed of in a timely manner, and "hot" trash was allowed to build up for weeks prio ing autoclaved. One former military aerobiology technician commented that had to clean Ivins' trash himself out of safety concerns. said that the civilians at USAMRIID did not take safety seriously. commented that when[___ looked at the agar plates that had sat in the biohazard trash bags for several days or weeks in 115, they were covered with bacterial growth. room| _was used to prepare the nebulizer and AGI's for exposures. erobiology Department personnel primarily used this room. Rooms [Jana [ _|were utilized to run the aerosol exposures. All of the aérosol challenges were run in room until December 000 whe first anthrax aerosol challenge was run in room . Room as subsequently used as_an additional Ames challenge lab. The challenges in room until December 5, 2000 used two hoodlines, titled hoods #1 & #2, for the exposure fter December 5, 2000 only the #1 hoodline was used in room Documentation shows that another hoodline, hood #8, in room was utilized for subsequent anthrax challenges. AEBROSOLIZATION PROCESS: The aerosol process was generally outlined in several Standard Operating Procedures (SOPs) used by the Aerobiology Department. The SOPs include: SOP Number Title omen md sed IND row