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To: Washington a. From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 Protocol D98-03 Title: Detection of Bacillus anthracis from Nonhuman primates after Aerosol Exposure Using Non-invasive Methods of Sample Collection Objectives: The objectives of this study are to a) acquire noninvasive samples from B. anthracis nonhuman primates exposed to aerosolized B. anthracis spores in order to establish what specimens and when during the first 24 hours B. anthracis organisms can be recovered and b) to determine the applicability of current molecular and immunological methods for detecting B. anthracis in these types of samples. 5/13/1998 Exposure 98-035, Protocol D98-03 PI; [ Tech: Dugway_Ba Ames Stock Room Hood 1, 8 NHPs exposed #10 LDsy- rroee ws) Na sD Ss oy rr Note: This is the first aerosol challenge utilizing the "Dugway Material" according to There is no entry in Ivins' logs to indicate bé that was given a sample of RMR 1029 or b7 that a Sample was given out to anyone in this time period. There is no documentation to verify that this challenge utilized material from RMR 1029 or from any other material linked to Dugway. However, it is documented that RMR 1029 was the only Ames spore preparation containing Dugway Ames located at USAMRIID prior to the mailings. RMR 1030 did not contain Dugway material. O ’ From 9/30/1998 through 8/17/1999 there were 10 anthrax aerosol challenges completed, however, non-Ames strains were utilized. All of these challenges listed either Ivins or as DIF the PI and of as the aerosol technician: was listed as having been involved with the challenge on 98. These challenges were done as part of Protocols number F99-07 and B98-03. The Ba strains used included Protocol D99-02 Title: Collection of Positive Control Specimens for Development, Validation, and Fielding of Diagnostic Assays for the Detection of Bacillus anthracis Objective: The objectives of this study are to acquire biological samples (blood, plasma, and serum) from non-human primates exposed to B. anthracis by aerosol route in order to: 12