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To: Washington m1 From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 be 7/10/2001 Exposure 01-067, Protocol _B00-03 —_ A i b2 bIF Ba Ames Stock RMR 1029 Room[__], Hood 8, 15 Rabbits exposed 7/11/2002 Exposure 01-068, Protoco = Ba Ames Stock RMR 1029 Room[ | Hood 8, 14 Rabbits exposed 7/12/2001 Exposure 01-069, Protocol BO0-03 a Ames oc Roon{___] Hood 1 & 8 29 Rabbits exposed Aerosol logs show that a total of 58 animals were exposed during this part of study BO0-03. 8/14/2001 Exposure 01-079, = PI: Tech: Ba Ames Registry No. 2244 Room[___] Hood 1, 40 Mice exposed 1 x 10’ cfu/ml, 1 x 108 cfu/ml, 1 x 10° cfu/ml, 1 x 10° cfu/ml a) sad GY a O Note: Antifoam was utilized as part of this aerosol challenge. There is no record in Ivins' logs to indicate that [____]was given any of RMR 1029-prior to 10/4/2001. The 8/14/2001 aerosol challenge was the last aerosol challenge utilizing Ba Ames at USAMRIID prior to the anthrax mailing on September 17, 2001. ANTIFOAM: Antifoam was used in aerosol challenges when the challenge material contained large amounts of protein. For this reason, antifoam was used more often with variots toxins and viral preparations rather than with bacteria. There were rare occasions that antifoam was added to the collison nebulizer in the challenges. It was added to the nebulizer if the nebulizer became foamy or gummy during a challenge. When antifoam was 20