◆ SpookStack

To: Washington mA From: Washington Field Re: 279A-WF-222936, 03/08/2005 b6 Fo Pe Purification of Bacillus anthracis Ames Spores for Aerosol Challenge!": Yy "Preparation of Bacillus anthracis Spores for Aerosolization"; an April 1997 SOP prepared by Ivins entitled "Quantitation of Bacillus anthracis Colony Forming Units"; an April 1997 SOP prepared by Ivins entitled "Preparation of Bacillus anthracis Spores for Testing Aerosolization Efficiency"; and a March 1997 SOP prepared by Ivins entitled "Preparation of Bacillus anthracis Spores for Aerosol LD50 Determination". In the folder, a Ba Ames Primary Subculture was shipped to Battelle in April 2004. Ba Ames ‘"ready-to-spray" spores were shipped to Battelle between April and September 2004. Furthermore | vine visited Battelle for observation and advice between May and September 2004. Notebook #16, also labeled #3464, contained _an entry by July 27, 1994. Ivins reported that of was performing work with Ames spores in rabbits. Ivins also attached an August 17, 1994 graph showing a gamma- irradiation kill curve for Ba Ames strain spores. The kill curve was produced by[ On_or about November 22, 1994, an experiment was to be conducted to and Ivins were to do the skin testing. Ba Ames was one the strains used in this experiment. ooo ee ws Oe)