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To: Washington ,@ From: Washington Field Re: 279A-WF-222936, 03/08/2005 md OS Cl or determine the virulence of some of their other spore preparations. Subsequently, Ivins attempted to i virulence of some ee Ames spon Experimental Protocol 1993, had an objective to written on December 8 written on December 8 Experimental -Protocol Experimental Protocol written on Jul comprised an Notebook 3465, issued September 16, 1992, included several protocols utilizing Ba Ames with as-yet unidentified individuals listed as principal investigators: Protocol written September_1 1992 1993, included a and Protocol written on May 11, Protocol written on December 9, 1992, include and Protocol [| written on April 1, 1993, included a This notebook also contained a Standard Operating Procedure for Lyophilization of Reagents, written February 24, 1992. Notebook 3269, issued 2 1990, listed an experiment Ivins performed for to determine whether anthrax spores germinate in R-medium or R-medium and 10% unheated horse serum. Ivins also gave some Ames spores to[ sion June 9, 1992 and on September 23, 1992. Notebook 1670, issued June 29, 1982, contained an entry written on March_3, 1983. Ivins gave Ba Ames concentrated supernatants cof | for LF and PA determination. Notebook 3080, issued November 20, 1987, contained information regarding an experiment conducted on or about January 21, 1988 byf and in which the two tested the Ba Aro strain as a vaccine against Ames spore challenges. On or about April 20, 1988, Ivins and[