Amerithrax — Part 9

75 pages · May 08, 2026 · Document date: Jun 2, 2002 · Broad topic: Terrorism · Topic: Amerithrax · 72 pages OCR'd

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To: Washington i@ From: Washington neal Re: 279A-WF-222936, 03/08/2005 also contained information on the efficacy of certain media on growth, concluding that agar was a better support matrix than bé agarose. b7c Notebook 1670, issued June 29, 1982, stated that on September 1 (year unknown) ——s—<“SC™t™t~—S.____] (tt As not known wherd ____]3s employed) gave Ivins B. thuringiensis, B. subtilis, and S. fecalis. The folder entitled contained a log of the amount of eceived, the amount used, and the dates of use. The log was begun October 27, 1997. The folder entitled "RMR 1029- Highly Purified Ames Spores - 3x107*" contained a set of e-mails between Ivins, dated between October 12, 2001 and November 9, 2001. The e-mails documented numerous individuals to whom the Ames strain was sent and the dates in which it was sent. . A folder entitled "Harvesting Spores - + GLP Spore From Dugway" contained "Acceptance Criteria Test Forms", which tested and plated RMR 1029 on March 18,.1998. The last page featured an e-mail sent from Ivins on January 17, 1997 to the followin rincipal investigators: In the e-mail, Ivins calculated the amount of cultures needed and the time it would take to produce enough spores for aerosol challenges of 1000 rabbits and 200 monkeys. Ivins concluded that the concentration of spores for each animal was based on what he and others in his group. administered (or tried to administer) to the monkeys and rabbits in F96-16 and F96-17, or 3.0 E9. Ivins prepared 8.5 milliliters of aerosol per animal, or about 8 milliliters per tube. Ultimately, Ivins calculated that it took 13 runs to generate about 3.0 E12 Ames spores for the "current batch". Since they. needed ten times that amount,:‘it would take them 130 runs with the flasks if performed with 2 liters per run, as they currently did. Therefore, it would take 130 weeks at one run per week or 65 weeks at 2 runs per week. The total amount of culture needed to produce the spores would be 260 liters. Below the email was Ivins' handwritten note describing what was needed, when, and in what solution the spores would be delivered. +

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To: Washington i@ From: Washington neal
Re: 279A-WF-222936, 03/08/2005

also contained information on the efficacy of certain media on
growth, concluding that agar was a better support matrix than bé
agarose. b7c

Notebook 1670, issued June 29, 1982, stated that on
September 1 (year unknown) ——s—<“SC™t™t~—S.____] (tt As not known

wherd ____]3s employed) gave Ivins B. thuringiensis, B.
subtilis, and S. fecalis.

The folder entitled
contained a log of the amount of eceived, the amount

used, and the dates of use. The log was begun October 27, 1997.

The folder entitled "RMR 1029- Highly Purified Ames
Spores - 3x107*" contained a set of e-mails between Ivins,

dated between
October 12, 2001 and November 9, 2001. The e-mails documented
numerous individuals to whom the Ames strain was sent and the
dates in which it was sent. .

A folder entitled "Harvesting Spores - + GLP Spore From
Dugway" contained "Acceptance Criteria Test Forms", which tested
and plated RMR 1029 on March 18,.1998. The last page featured an

e-mail sent from Ivins on January 17, 1997 to the followin
rincipal investigators:
In the e-mail, Ivins calculated

the amount of cultures needed and the time it would take to
produce enough spores for aerosol challenges of 1000 rabbits and
200 monkeys. Ivins concluded that the concentration of spores
for each animal was based on what he and others in his group.
administered (or tried to administer) to the monkeys and rabbits
in F96-16 and F96-17, or 3.0 E9. Ivins prepared 8.5 milliliters
of aerosol per animal, or about 8 milliliters per tube.
Ultimately, Ivins calculated that it took 13 runs to generate
about 3.0 E12 Ames spores for the "current batch". Since they.
needed ten times that amount,:‘it would take them 130 runs with
the flasks if performed with 2 liters per run, as they currently
did. Therefore, it would take 130 weeks at one run per week or
65 weeks at 2 runs per week. The total amount of culture needed
to produce the spores would be 260 liters. Below the email was
Ivins' handwritten note describing what was needed, when, and in
what solution the spores would be delivered.