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Amerithrax — Part 10
Page 122
122 / 234
ALL IHFDPEATIUH
CONTAINED
' HPEIN
I3 UNCLQSSIFIED
. mm
12-1;-">:|>:|a BY
"U354 1
5»
7. lV-B
Bentonite feed stock
G D
u Wm
In
no growth seen <1.2 X 105 CFU
per
gram!
Visual inspection of suspensions of the material under phase contrast
microscopy demonstrated very poor preparations of spores. The preparations
were all highly granular and did not easily go into suspension in sterile water for
injection. Individual preparation observations:
Samples:
1. Vlll-B
21 June 95 ~ Dried BT powder - very granular when
suspended; many spore clumps; numerous non-refractile spores; not a good
preparation at all; fewerthan half of the material consists of refractile spores
2. Vll-B
Spray drier - BT - very granular when suspended; a great
deal of large clumps of spores and debris; about 2/3 of the material are refractile
spores
3. l-B
Dried powder - very granular when suspended; a lot of
debris; no
refractile
or
non-refractile spores seen
4. IX-B
Spray-dried BT - very granular when suspended; many
spore clumps; numerous non-refractile spores; not a good preparation at all;
fewer than half of the material consists of refractile spores
5. Vl-B
Dried powder from fermentor run - very granular when
suspended; many spore clumps even more than in preps 1 and 4!; numerous
non-refractile spores; not a good preparation at all; fewer than half of the
material consists of refractile spores
6. V-B
21 June 95 - Dried powder from spray drier - very granular
when suspended; many spore clumps even more than in preps 1 and 4!;
numerous non-refractile spores; not a good preparation at all; fewer than half of
the material consists of refractile spores
7. lV-B
Bentonite feed stock - very granular when suspended; a lot
of debris; no refractile or non-refractile spores seen
interpretations and conclusions: lf these are preparations of bacterial
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