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o FD-302a (Rev. 10-6-95) S , S 279A-WF-222936-USAMRIID, 279A-BA-C101392 Continuation of FD-302 of ee ,On 06/06/2003 bleach. Swipes of biosafety cabinets following decontamination yielded no growth on culture media, but detectable levels of pxXOl and pxO2 in some samples. A study was conducted whereby pre- decontamination sampling was used to identify the 'hottest', or most contaminated, areas in a biosafety cabinet. Detectable levels of DNA were obtained from these areas after decontamination with bleach. , Page The following USAMRIID personnel may provide further insights into the impact of various decontamination methods on subsequent scientifi : UCE IVINS and Internet searches may yield additional information related to decontamination of biological materials, including diagrams of key mechanisms of inactivation. [| provided the Agents with a manuscript containing an overview of decontamination technologies and descriptions of the mechanisms of action of numerous decontaminants on micro isms. The document, titled DECONT = ored by |_| and SCIENCE APPLICATIONS INTERNATIONAL CORPORATION (SAIC), NATIONAL CANCER INSTITUTE AT FREDERICK, FORT DETRICK, MARYLAND, 21702, telephone The manuscript is maintained in. a TA envelope (FD- ~ 340) lso recommended the following textbook as a useful reference: DISINFECTION, STERILIZATION, AND PRESERVATION, 5th edition, Seymour S. Block, Editor, Lippincott Williams & Wilkins, Philadelphia, Pennsylvania, 2001. Photocopies of the first two pages of the textbook were provided to the Agents and are maintained in a 1A envelope. The Agents were also provided with copies of USAMRIID'S records of transfers of Bacillus anthracis that occurred from 1997 thr quested in a letter dated June 5, 2003, from SSA The EA101 forms provided by USAMRIID are maintained in a 1A envelope. ion 3