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FD-302a (Rev. 10-6-95) 279A-WE-222936-USAMRIID bé bIC Continuation of FD-302 of i ,On 03/09/2004 __, Page 2 the aerosol preparation laboratory where the nebulizers and all glass impingers (AGI) were prepared. Room contained the aerosol hood line. No other rooms were use or aerosol work with )& Anthrax_in Building noted that the door between ?7° rooms d Ww closed by screws put into place on — the room side. said that in 1995, Ames work was , done in suiteL_]of Buildin at _USAMRIID while renovations were being done to Building recalled Ames aerosol challenges being conducted there during that period in suite Ames used in the challenges was grown in suite at USAMRIID. The we b7C per aerosol enge. The conical tubes were kept in the refrigerator in room — [ of Building | |noted that if any conical tubes were missing from room someone would notice, however, no one would notice if liquid was taken out of the tubes. BRUCE IVINS would place tape on top of the conical tubes, however, the tape could be easily replaced. No agents for the aerosol challenges an in the walk-in cooler on the first floor of Building At the end of the aerosol challenge approximately six (6) or seven (7) ml of B.a. would remain. The B.a. that was not used in the challenges was normally autoclaved prior to being removed from the hood. The remaining material, except for the AGIs, that was used in the challenge would be placed into a bag and autoclaved at the end of the day. The AGIs were sprayed with bleac jor to being removed from the hood line, and then taken to room for plating. After the plates had been left in the incubator overnight, they would be completely covered with growth. The plates were then read in the morning following a challenge. After the plates were read, they were placed into a bag and clearly marked with what pathogen was in the bag, what strain the pathogen was, and who the investigator was. The bags would remain in room until nearly overflowing, or until the number of bags_in the room became an obstruction. The bags often sat_in room for several days or weeks prior to being removed. noted that) was fascinated with how much growth appeared on the plates after several days or weeks. The bags were then taken to the basement to be autoclaved. The bags were placed into metal garbage cans in the basement, next to the autoclave, in case there was a leak in the bag. The animal caretakers at USAMRIID would autoclave