◆ SpookStack

p ALL E INFORMATION CONTAINED & HERE i UNCLASSIFIED DATE Lle-10-2008 BY 60224 U0 BAM/SRS/LSec SPORE PREPARATION FORM STUDY #: B00-03 (part 2: 1 dose efficacy with 25 pg PA; 2 preparations) DATE: HJUE 00 SPORE PREPARER: Ivins —_ 171 July 00 bIF SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10" /ml DESIRED CONCENTRATION OF SPORES FOR AEROSOL: DILUTION FACTOR: 1 ml of spores per [jf of aerosol suspension NUMBER OF ANIMALS TO BE CHALLENGED AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR AEROSOL: | | AMOUNT OF SPORES ADDED TO BOTTLE] AMOUNT OF WATER ADDED TO Borin] | = | 1 duly 00 SPORES DELIVERED TO BE AEROSOLIZED AT: merning-of 11 Juls-00 PLATE COUNTS (FROM| _ PILUTION): PLATE 1 PLATE 2 PLATE 3 PLATE 4 PLATE 5 AVERAGE= 30 CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE ORINITIALS: 2. DATE: /€3,/00 DATA VERIFIED | DATE: Hf ws Lo