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AlgegenT INFORMATION CONTAINED ®@ t TS UNCLASSIFIED r fa ata 6 DATE le-L0-20068 Q324 UC BAW/SRS/LSC SPORE PREPARATION FORM STUDY #: D99-02 DATE: 14 SEP 99 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames, RMR 1029, in 1% phenol pe APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 2.5 X 10°/ml DESIRED CONCENTRATION OF SPORES FOR AEROSOL: | DILUTION FACTOR: | ml of spores per [| of aerosol suspension NUMBER OF ANIMALS TO BE CHALLENGED[__ | AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR azrosor{ | AMOUNT OF SPORES ADDED TO BOTTLE, —_| AMOUNT OF WATER ADDED TO porte, | SPORES DELIVERED TO BE AEROSOLIZED AT: 0730 . “4/23/65 PLATE COUNTS (FROM DILUTION): PLATE1 PLATE2 PLATE3 PLATE4 PLATES * Oo “ AVERAGE=. & 5.4 CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE OR INITIALS: Be DATE: 9/2266 . — / 2 3/99 bs DATA VERIFIED BY: DATE: 7/3 | 4