◆ SpookStack

mn ALL [ ORMATION CONTATNED * j HERE 3 UNCLASSIFIED ‘ DATE la-lO-2008 BY 60324 UC BAW SRS /LSc SPORE PREPARATION FORM STUDY #: B00-03 (part 1: 1-dose efficacy test with 2 PA prenarations} DATE: 5 April 00 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10%/ml DESIRED CONCENTRATION OF SPORES FOR aerosol; | DILUTION FACTOR{ _ lot spores per [he aerosol suspension be b7F NUMBER OF ANIMALS TO BE CHALLENGED| —_| AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR srrosor| | AMOUNT OF SPORES ADDED TO BOTTLE| | AMOUNT OF WATER ADDED TO BOTTLE{ | SPORES DELIVERED TO BE AEROSOLIZED AT: morning of 5 April 00 PLATE COUNTS (FROM|__ pILUTION): PLATE 1 PLATE2 PLATE 3 PLATE 4 PLATE 5 2) 19 /3 ZR = BS AVERAGE = Jif CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE OR macs} | pate: 4/2 / rv DATA VERIFIED BY: p we pate: $2 [60 sal omen