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* on °@ e FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID Continuation of FD-302 of a ,On March 20, 20 (fee 2 volunteered[___] thoughts concerning the anthrax powder contained in the mailings and explained that investigators should be focusing on the chemistry of the anthrax. [explained that spores normally tend to stick to each other but with the anthrax powder, the spores had to have been separated. In order to accomplish this, a positive or negative charge had to be introduced, a hyperphobic or polar molecule, like a dry detergent, and the Liled during growth. The material must be charged and polar. believes that the use of a mass spectrometer would aid in identifying the chemical make-up_of the anthrax. indicated that if asked,[_] would volunteer| | assistance in aD) omen ~l O croor ~1 explained housed_a 100-liter fermentor known as "Big Bertha" | there were two separ 100-liter fermentors known as "Big Bertha" in Building USAMRIID i 19 . obtained these fermentors around 80-1985 could best a a historical of the fermentors. [____Jexpiainea that fermentors were primarily used back in the 1980's but thei se drastically declined due to the advent . of molecular cloning. advised that a bioreactor is similar toa jj... or and that it is possible to grow Ba using a bioreactor. was given an opportunity to review a document_titled a Inventory Check and Corrections: 8 Nov 2005. two Braun liter fermentor was purchased in 1983. Biotech fermentors, stock numbers [_|"Big Bertha" is the 100-liter fermentor identified as stock aamiberfo on the sa sheet and believes the