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FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID Continuation of FD-302 of ee ey ce On 05/01/2006 __. Page 2 iers at USAMRIID named did not know former USAMRIID [ ___contizmed that, while at USAMRIID,[ _|did some research work with Bacillus anthracis (Ba) and belj s it was the Ames strain but was not one hundred percent sure. confirmed working with Ba spore preps, always obtained from[___] believed that obtained the Ba from a freezer located in th "hot suites" and that has previously provided a more accurate and detailed account of research work at USAMRIID to investigators. advised that advised that this was the general standard practice at USAMRIID. [jexplained that did not conduct any research utilizing a fermentor nor was involved in any research pertaining to aerosol challenges or dry work. was not aware of any dry work being conducted at USAMRIID. was unaware of any plans in the future to conduct dry work at USAMRIID. [__]advised that weaponizing Ba would be a big undertaking and a lot of_effort. did not know how someone would go about doing that and would not know what type of equipment would be needed to aerosolize Ba_other than it would involve more than just a freeze dryer. did not use a lyophilizer while at USAMRIID but on a rare occasion or two, [_|saw the lyophilizer being used for protein studies. [___Jexp1ained that while at.USAMRIID,[[]|did on occasion use an autoclave or place materials in an autoclave for someone else to autoclave at the end of the day.[- si recalled that there were two "Pasteur" autoclaves located in the hot suites and that it would_take approximately one hour to complete an autoclave cycle. [—_Jadvised that when working with cultures[__] also would add bleach to the cultures prior to having the cultures autoclaved. Employees could not leave cultures out in the suites at the end of the day.[__jwas not aware of any special or additional security measures that were in place when working with Ames Ba. was ver icky about the quality of spores rrr s IN ~1 O Hy fe