◆ SpookStack

“ . ‘ s - ° FD-302a (Rev. 10-6-95) : 279A-WE-222936-USAMRIID, 279A-BA-C101392 Continuation of FD-302 of | | ,On 04/29/2003 _,Page = 2 obtained wet B.a. Ames spores for the___] experiments from IVINS. IVINS' laboratory grew the spores in Leighton Doi liquid media, using a shaker and a three day incubation period in the dark. en ~~) oN a ID b6 b7c bUF At the conclusion of the experiments, autoclaved the B.a. Ames spores[ _jobtained from IVINS. The spores for the project were obtained from IVINS and were grown in LB broth at 37°C in a small flask. Antibiotics were used to select clones with the GFP plasmid. The Ames stock obtained from IVINS was autoclaved. [made spores using non-virulent B.a. strains. The production method depended on the strain being used. SBA plates were, used most often; however, B.a. Sterne was grown usin