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ED-302a (Rev. 10-6-95) 279A-WE-222936-USAMRIID be PTC Continuation of FD-302 of oe ,On 04/17/2003 __. Page 2 The predominant request was for B.a. Sterne, an avirulent strain, as this was a commonly used control in experimental research. lled sending both Ames and Vollum B.a. strains on occasion. could not identify the facilities that requested B.a. Ames. was funded through the ARPA) and materials were oft to other facilities under the same DARPA program. ecalled the frequent exchange of materials with the UNIV ABAMA, UNIVERSITY OF MARYLAND and a facility in CHICAGO. as not sure whether these exchanges included B.a. Ames, but indicated that the UNIVERSITY OF MARYLAND did not have Ames, nor the BSL laboratory capabilities to handle that type of pathogen. primary stocks of myria a, Strains were main bé Special Pathogens Branch[ __] suite in Building enerate DIC a vial of material for transfer to another facility, b2 bTE Typically, matched exactly the requested amount. Since B.a. Sterne was requested most frequently, extra vials of secondary stocks were sometimes prepared to fill future requests. Due to the special packaging requirements of temperature sensitive materials, the procedure used to prepare requested materials for shipment was modified. Modifications included qc preparing multiple aliquots in excess of the amount requested and lyophilizing of these secondary stocks. The lyophilized materials could be maintained in the refrigerator or at room temperature. Transport was h ditional temperature requirements. b6 b?7c 22 bUF used to dry samples for shipment. One lyophilizer was under