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i FD-302a (Rev. 10-6-95) @ @ bo 279A-WF-222936-USAMRIID buC Continuation of FD-302 of ee ,On 04/07/2004 __,Page 2 Lsaia that the same AGIs have been used for at least the past ten (10) years. Recently the AGIs have been modified to use a screw top instead of a push on-top. The material that was collected in the AGI would be maintained for several days or longer following a challenge. Small amounts of the antifoam were aliquoted into tubes. In previous years, larger tubes were used which caused excess waste. noted that Anthrax spores are very resilient. [_ jis unaware of any antifoam stock ever reported missing. [_|saia tnat[ would assume that[ | new the process of using antifoam as part of the aerosol challenges. in the past olive oil was used for aerosol challenges. The olive oil was used for challenges involving toxin proteins. Olive oil has not b j 0) years. as not seen a bottle of olive oil at USAMRITID since the early 1990's. [stated that there is no difference in the post challenge assays if olive oil or antifoam are used during the aerosol challenge.