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* RD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID; 279A~BA-C101392 . 1 pIC Continuation of FD-302 of i ee On 24 /24/2003 3 , Page the of USAMRIID prior to workin for[ | [| has been in aboratory for approximately years. Notebooks for the research conducted by[ _Jana technicians are kept inside the hot ed and faxed to the cold side. Much of the information and data has are kept inf] computer. Bacterial contaminations in Building|__]nave occurred twice to Knowle few months ago, routine surveys of the door handles in the floor of Building were found to bé be contaminated with B.a., although the strain was not identified.. b7c The cause of the contamination was traced back to poor b2 decontamination procedures by some employees who work on aerosol bvE challenges. This issue was resolved this past November. The other contamination issue was discovered 6 to 9 months ago when a laboratory technician had a routine test conducted by come up positive for contamination. What was first thought to be: the result of poor laboratory practices by the technician later turned out to be a contaminated container that caused the cross-contamination. It is believed that this contamination occurred about 1 year ago. The Standard Operating Procedures (SOPs) for decontamination calls for the use of bleach. Everything in the laboratory is wiped down with bleach, which can cause damage over time because of the corrosiveness of the substance. Items such as plates, flasks, and other smaller equipment are autoclaved after it is cleaned with bleach. People who work in the animal rooms wear: hoods, tyvec suits, and 2 pairs of gloves. The protective gear is removed upon exiting the animal rooms and all employees step onto a small puddle of bleach to decontaminate their shoes. Animals who die in the laboratory are placed in a bag, sprayed with bleach, brought into the cold side, autoclaved, and then disposed. does not have access to the Virology suites in be Building pre When USAMRIID received the was responsible for It took approximately determination and was in USAMRIID for e entir uration of the received a subc the material Erom to analyze. The material was brought to in powder form in a plastic bag From [took a letters, to make that