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. e RET ® FD-302a (Rev. 10-6-95) SEsREL Continuation of FD-302 of Bruce Edward Ivins £ 279A-WF-222936-USAMRIID Dugway-produced spores and spores made by IVINS, was kept only for the purpose of allowing the researchers to estimate how much of the material was left so they would not run out of spores for aerosol challenges. The record was not kept as any kind of precise inventory for security reasons. The amounts of remaining material were only estimates and were not accurately measured for each entry. The entry on tvins'{ | record for Protocol B97-05, dated March 23, 1999, documents the first time that[ was ever taken to USAMRIID building 1412. The study was not an aerosol challenge, but was an injection challenge on guinea pigs. Only approximately one (1) ml was taken to 1412 at that time. IVINS thinks the rooms used in 1412 for the injection study were [7 Jand The entry on the record for dated September 17, 1998 was for a characterization and outgrowth study comparing spores which were germinating with vegetative spores. The first aerosol challenge which utilized was conducted on April 3, 2000 and was the first part of Protocol BOO- 03. ‘ IVINS mentioned the recent issue discussed with SA regarding the concentration of RMR 1029 when it was returned To IVINS from the FBI. IVINS had gone back through his documentation after talking with SA and had listed the concentrations of RMR 1029 at various points in time since its creation. The concentration over time ranged from 3.1-5.2 x 107° spores per ml. When the sample was returned to IVINS from the FBI, he found the concentration to be 2.4 x 10° spores per ml. For the concentrations determined by IVINS, he swirled the flask of RMR 1029 to mix the spore preparation, removed a subsample and prepared dilutions, then plated the 107? dilution. He then counted the colonies which grew from this dilution. The decreased spore concentration of RMR 1029 after its return to IVINS was not a major problem. They readjusted the sample by adding more spores to it so there was enough Ames material for[._ +) vaceine challenge. IVINS found it interesting or ironic that had an Ames sample labeled The spores were actually made in 1993. SESREL ,on 1/13/2005 » Page 3