◆ SpookStack

_Deschle ALL [ OW CONTAINED ATI HEREIN [5 UNCLASSIFIED ‘ DATE lLe-08-2008 BY 60324 TC BAW/RS/LSc Analysis of Sample Date analyzed - 17 October, 2001 Date of Report - 18 October, 2001 Sample SPS02.57.03 bé | received the sample (in ziplock bags) from[__——SSSSS—sdon' the RIC afternoon of 17 October, 2001. The sample was taken into B-3. Insufficient b2 powder was on the letter, so powdery material was scraped from the envelope BTE and put into a small, tared, glass container. The container was reweighed and the net weight of the powdery material was determined to ref ot material was eds sterile water for injection to make a total of (and approximatel of suspension. Ten-fold dilutions were plated out onto TSA, then incubated overnight. Plate counts were made, and it was determined that the original tube contained Since there was[____| [_Jof material, this calculates to b of powder material. Visual inspection of the suspension of material under phase contrast microscopy found no visible vegetative cells, no visible debris, and very few small clumps. Most of the material appeared to be individual refractile spores. Interpretations and conclusions: If this is a preparation of bacterial spores, it is an extremely pure preparation, and an extremely high concentration. These are not "garage" spores. The nature of the spore preparation suggests very highly that professional manufacturing techniques were used in the production and purification of the spores, as well as in converting the spores into an extremely fine powder. Bruce E. Ivins, Ph.D. USAMRIID Bacteriology Division