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FD-302 (Re¢. 10-6-95) DATE la-00-201 Fp) ALi NFORMATION CONTAINED av © Hi IS UNCLASSIFIED w = : -1- FEDERAL BUREAU OF INVESTIGATION Date of transcription 1/31/02 On January 23, 2002, BRUCE IVINS contacted Special Agent of the Federal Bureau of Investigation (FBI) at the United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Maryland, regarding recently-obtained information about Bacillus anthracis. IVINS provided two color digital photographs of Bacillus anthracis growing on agar plates and also provided the following information: Ivins(f si orrk with the oldest and most original culture of the AMES strain of Bacillus anthracis at the AMES by transferring it from plate to plate rather than going back to original sample. The transfers, sometimes referred to as serial dilutions or passages, cause genetic mutations. It appears that the mutations may have resulted in differences in morphology between “stock” of AMES and the original USAMRIID- AMES. The photographs provided by IVINS show the two “stocks” (the original ana[ at approximately 42 hours of growth. The i morphologica erences are not apparent until after more than 24 hours of growth. The differences include_a_qrainy-like whitish growth and border variations in the “stock” which are not visible in the original USAMRIID “stock” of AMES thus far. USAMRIID also has a stock of AMES spores made for them at Dugway Proving Ground. There could be other stocks of AMES at USAMRIID, but IVINS does not know _ of any more than the three. IVINS does not have access tof JAMES which is maintained IVINS has all of the records of transfer f the original USAMRIID stock of AMES. Even if transferred some of it, IVINS would still have the record of the transfer. IVINS feels his records of the transfers are very accurate. The AMES stock from the original slant was sent to_Duqway Provin Ground, Battelle Memorial Research Institute, D.R.E.S (the Defense Research Establishment at Suffield, Canada), and _to the University of New Mexico. Samples from collection were sent to Porton Down, who sent [| Investigation on 1/23/02 Fort Detrick, Maryland . = File# 279A-WF-222936 ~ Ga Date dictated 1/25/02 by This doc nclusions of the FBI. It is the property of the FBT and is loaned to your agency; y' it and its contents are not to be distributed outside your agency. [ O BY 60324 UC BAW SRS /LSC Dé bIc on ad 3 a b2 b?E bo b7c