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FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID Continuation of FD-302 of Bruce Edward Ivins ,0n 05/07/04 , Page 3 The Ames produced by Dugway for USAMRIID in 1997 was made for aerosol challenges and was widely used at USAMRIID by multiple researchers. IVINS kept a record of his distribution of this material. He had previously provided his record to agents, but he has updated the distribution list since then. IVINS provided his b6 updated list during this interview. SSA[__]|pointed out a b7c mathematical error on the list to IVINS. He had not previously noted the mistake and believes there should thus be more material left than his balance shows. When challenges were scheduled and required the 1997 Dugway Ames, the bottle of Ames spores was taken to either Room[_| or of building 1412. Sometimes it was taken there a day or two pz prior to the scheduled spray. The aliquots of Ames were prepared b7E in 1412 from the main bottle. The dilution tubes made from the Ames collected by the all-glass-impinger (AGI) were plated on tryptic soy agar plates. These dilution tubes of Ames were stored in the first floor cold room until the plates had been counted. Sometimes the tubes might stay in the cold room for 24 hours to several days following a spray. This cold room is a walk-in refrigerator on the first floor, not in the basement of 1412. Other than this Ames material temporarily stored in 1412, the larger quantities of Ames were kept in suite B3. The B3 material was not under lock and key, but_was kept inside the hot suite. It was March 1999 when Ames sample[__Jwas first taken into building 1412. has used _ IVINS Ames material known as RMR 1029. obtained of this material from IVINS on 8/28/2000 for diagnosis detection research. LO worked wie fo so if IVINS gave Ames to him, it would have been for aerosol challenge work. IVINS believes all aerosol challenges with Ames at USAMRIID from 1997 until 2002 utilized the Dugway Ames. IVINS does not believe he provided a sample of RMR 1029 to for[____—sd| collection. IVINS. was the one who usually disseminated the Ames as people requested it. IVINS does not recall ever seeing any researchers from DRES inside suite B3 of USAMRIID. Though the post-heat-shock plating is done in 1412 to determine the spore concentration after heat-shocking, other plating is done inside suite B3 of 1425. Whenever IVINS' research group wanted to determine the concentration of any of their supplies of spores, they plated Ames in suite B3. These plates, once counted, were autoclaved out through the suite. They were put in autoclave bags which were tied