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@ r ° 5 , FD-302a (Rev. 10-6-95) 27 9A-WF-222936-USAMRIID Continuation of FD-302 of Bruce E. Ivins ,On 09/08/2004 Page 1030 was produced in Leighton and Doi broth, in accordance with UIB-BI- 3. IVINS advised that he would need to review his notebooks to b determine what seed stock was sent from USAMRIID to Dugway in 1992. bic IVINS explained that Capsule Agar detects capsule formation in Ba. He noted that Ba would not show capsule formation when grown in carbon dioxide at less than 5% unless both plasmids were present. IVINS advised that the material sent from Southern Research Institute (SRI) to California by recently was grown on caps phen O qed 2 ANUS pe Proced = e date originated 3/23/97 and effective date 4/7/97. prepared by i is Maintained in an FD-340 in the 1A section of the file. This documen IVINS has worked with Covance in Denver, Pennsylvania. USAMRIID wanted Covance to produce antiserum for some spore antibody studies. These studies were performed ee In support of some Ba vaccine studies at USAMRIID, USAMRIID has provided the Ba vaccine to Covance. Covance immunizes the rabbits, and the rabbits are then brought down to USAMRIID for challenge. IVINS is not aware of any live Ba used by Covance. All Ba provided by USAMRIID to Covance is gamma-irradiated. is doing some work with Covance and IVINS works with The only Covance facility visited by IVINS is the one in Denver, Pennsylvania. , Regarding the sample sent to the Defense Research Establishment Suffield (DRES) in Canada, IVINS believes it was vegetative cells sent by. He believes this shipment was not spores and was probably sent in a frozen state. IVINS noted that this information could be found on the form 11R. He does not know the origin of the sample sent to DRES. IVINS noted that ood at making spores. He also added that provided a one-page e-mail at DRES, dated 4/8/04, regarding this Ames Ba spore shipment .to DRES. This document is maintained in an FD-340 in the 1A section of the file. Regarding sample 7739A, grown by [|__| in, 1997, IVINS did not what(7——s—édS: used to make this sample and stated he would have to check his notebooks and ask around to determine what may have been used. IVINS described heat shocking of spores. He explained that it was an activating mechanism used to synchronize spores prior to