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Yo +1 Oo Od UL w FD-302 (Rev. 10-6-95) ALL INFCREATION CONTAINED HEREIN IS UNCLASSIFIED -l- DATE le-l6-2000 BY 60324 UC BAW/DE/TH , FEDERAL BUREAU OF INVESTIGATION Date 6f transcription 09/10/2004 BRUCE E. IVINS, pr Ncipal Investigator, date of birth Be telephone number Was interviewed at bic 04/24/1946, his place of fe) U Institute of Tnrectious “Disease (USAMRIID) ha Fort Detrick, Maryland. After i 2 caetncte of the identities of the interviewing agents and the nature of the interview, IVINS provided the following information: In 1985, IVINS was concerned about the possibility of genetic variants of Bacillus anthracis (Ba) making their way into cultures which were grown by sweeping batches of spores off of a seed stock and plating these spores. IVINS noticed that when he took a full sweep of many spores to inoculate a plate (sweep technique), he would get a more heterogeneous culture. In addition, when he passed a culture that was plated as bé described above, each generation of passage would include more bi aberrant colony morphologies. When IVINS used just one colony to inoculate a plate (single colony technique), the result was a more homogeneous culture with just a few aberrant colony morphologies. Although he was advised by the "old timers" like that he should inoculate plates by taking a sweep of spores from the seed stock, IVINS believed that growing spores in this way was contrary to pure microbiology culture technique. As a result, in 1985, IVINS dipped into the original 1981 Ames slant to begin a new seed stock which he refers to as the "1985" sample. IVINS intended to use this sample as a seed stock so that he would not have to continually dip back into the "1981" original slant. He did not recall the sample name, number, or what was written on the tube containing the "1985" sample. He does not think that any of the "1985" sample remains, however, if it does,’ the FBI has it in their repository. IVINS cannot recall if he used just one spore ‘from the "1981" sample to create his "1985" seed stock or if he used just a few "normal" spores. IVINS noted that there were originally two "1981" original slants; however, there is now only one "1981" slant. IVINS does not know what happened to the second slant. The "1985" sample was being used as seed stock for intramuscular (IM) challenges, therefore USAMRIID was not growing large batches of spores. Large batches of spores are Investigation on 09/08/2004 at Frederick, Maryland -” Fie# 27 9A-WP-222936-USAMRIID ~ 4 45 Date dictated 09/10/2004 Saline This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loan ake your agency, it and its contents are not to be distributed outside your agency. >