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FD-302a (Rev. 10-6-95) 279A-WF- 222936 Continuation of FD-302 of | | ,0n 12/03/2001 ,Page 2 5. Were your strains fully characterized? Answer: Yes. Our Ames produces toxin (has both plasmids to form the toxin and forms a capsule). 6. The question was omitted from the submitted protocol. 7. What is your recollection of the earliest isolation/identification of the Ames strain of anthrax? Answer: earliest recollection of the Ames, was when 8. Do you know of any commercial sources of the Ames strain? Answer: ‘[ si was fairly certain that anthrax could have been obtained from the American Type Culture Collection (ATCC) which was originally in Rockville, Maryland but moved to Virginia. However, since the new law went into effect they will not provide any select agent materials. In our modern environment no. one would indiscriminately pass around anthrax cultures. That said, if a legitimate organization; certified by the CDC with a legitimate need, Dugway would provide a culture, and the CDC would receive a confirmation receipt of transfer. Everyone would have to follow all the rules. 9. If you or your facility have the Ames strain, would you provide isolates for analysis? Answer: Yes, under the above described conditions. 10. Do you collaborate with other Laboratories? Which ones? How? — — — = —— = vaccine —— 11. Is Bacillus anthracis routinely shared between labs? How? Criteria for sharing? Answer: We have sent live anthrax to Battelle. See question 8. 12. Who are the individuals or organizations working with anthrax as a select agent? List? bé bi7c