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Amerithrax — Part 32
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Continuation of FD-302 of fo ton 09/04/2002 »Page_ 3
Pir time consuming as they take place over three consecutive days.
FD-302a (Rev. 10+6-95)
279A-2F-222936-SUH__———«4dit 302
2
thought that the spray, along with the surroundings of suite
would have been fascinating to
Upon exiting theL__ J wourld_have
been able to see the preparation for an anthrax spray in and
would notice the activity as
[venta often observe anthrax sprays and no one wou other
as it was a very hectic atmosphere during the experiment. Only
a mask and scrubs had to be worn to be in the lab during the spray,
and it would be easy to gain access to the lab as the combination
lock would be disabled in order to expedite the set up procedure.
asked questions about the dosage and media being used in
the sprays.
BRUCE IVINS would culture the B.a. used
in the aerosol experiments in suite IVINS would need to leave
in order to pick up the samples from Bacteriology, and at_that
time the lab was left unsecured. Further, the door between[ _Jand
was left unlocked, and there was a refrigerator in[___]that did
not lock.
IVINS prepared the B.a. for the nebulizer in Buildin
| lana
[se He would then bring the B.a. to Building suite
transfer the samples to 50 milliliter (ml) conical tubes. The
samples were_then sent straight to the hood line for the aerosol
challenge. [___———__—sirecailea that IVINS labeled everything and
would tape the tops of the tubes for easy removal.
After the sprays, the samples would go back to IVINS in
15ml conical tubes to be transferred to an agar plate and left in
an incubator overnight in[___] This was done to quantify the
amount of B.a. in the samples that had been used.
advised that[__]would not have noticed a missing sample, but
perhaps IVINS would have. Any remaining labeled samples would have
been kept in the unlocked refrigerator in[___] Other remainin
tubes and plates would have been in the unlocked incubator in ro
These tubes and plates would not have been strictly accounted for.
Once the tubes and plates in the incubator had been
checked for colonies, they would have been_placed_in a garbage bag
under one of the hoods to be autoclaved. CT
they could have sat in the bag for weeks.
advised that
be
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