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To: Washington Field From: New Orleans Re: (U) 279A-WF-222936, 03/04/2002 organism uses the various substrates. Based on the study, four groupings of anthrax were identified. bé i oe In a couple of the studies atL__| the Ames was — (T}- passaged, but only_a few times. The Ames is stored on protect bI7D beads made by al The beads are ceramic and have a cryoprotectant on them. Each time Ames is needed for a project, one of the beads is” removed and used. On the inventory, Ames is listed e listings are for numerous protect many times, but the multip beads made with the original USAMRIID Ames. The dilutions listed on the inventory are of vollum. Toe understands that Ames is endemic in Texas. believes Ames originally came from Texas. In the work conducted at[___]} Ames is grown on agar plates, but does not have experience d isa came to not oes E4 as the expertise to prepare aottsrs ry or powdered work except for Dugway's teers, [1 provided a copy of an advertisement for a a bench- top grinder whic thought might be the type of equipment used to prepare the letters. ; Just prior to septenber 11, Bh study to. determine how easy it would be for someone to grow the B. anthracis Ames strain at home outside a laboratory. They used old recipes and media formulations and bought plies at a grocery store. The Ames grew very well. _ SECRET _ 10