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To: Laboratory From: Washington Field Re: 279A-WF-222936, 11/01/2005 threshold for Bacillus globigii contamination of which, if exceeded, should call for aborting and autoclaving the production. Summary of Samples Recovered Samples labeled with dates consistent with production runs form the project entitled "Procedure for Anthrax Spore Preparation in Bench Top Fermentors" were recovered in a consensual search of the Lothar Salomon Life Sciences Test Facility at the Dugway Proving Ground, Dugway, Utah on 06/30/2004. Irradiated samples bearing dates 04/12/1997 (0.5m1), 04/14/1997 (0.5m1), 04/22/1997 (0.3m1), 05/22/1997 (0.25ml1), 07/11/1997 (0.25m1), 07/17/1997 (0.5ml1), and 07/21/1997 (0.25m1) were all recovered from the above search. One (1) sample bearing the date 08/03/1997 (0.25ml) was also recovered in this search, however, this date falls directly between production runs from 08/02 and 08/04, and therefore may or may not be correlated with the material shipped to USAMRIID for this project. Additionally, 19 scanning electron microscopy (SEM) stubs were also collected during this search. Ten (10) of SEM the stubs can be associated by dates with production runs from this Dugway/USAMRIID project. SEM stubs correlated with production dates 06/28/1997 and 08/04/1997 were not recovered. All SEM stubs that were recovered and believed to be associated with this production project were analyzed using SEM/energy dispersive x-ray spectroscopy (EDS) by the FBI laboratory's Chemistry Unit, Quantico, Virginia. No silicon, tin, or iron signatures were identified in these specimens. Of the material recovered from these searches, no live spore material bearing production dates from this Dugway/USAMRIID project was reclaimed. It should be noted that there are erroneous calculations and inconsistencies in volumes when comparing notebook with the Quality Assurance/ Quality Control paperwork affiliated with these production runs. Understanding these inconsistencies along with the level of Bacillus globigii contamination in materials shipped from Dugway to USAMRIID is eritical for furthering investigation into genotypically compelling anthrax materials at USAMRIID. - Based on genetic and forensic signatures pertaining to the RMR 1029 spore material, further exchange with Amerithrax investigators and[ | may be warranted. o¢ [_0505a.ec 5