◆ SpookStack

M !. To: Laboratory From: Washington Field Re: 279A-WF-222936, 11/01/2005 before and after heat shock procedures no Bacillus globigii contamination was noted. On 06/23/1997, laborted the fermentation run that b6 began on 06/19/1997 due to Bacillus globigii contamination. b7C According to notebook this batch was autoclaved (contamination and autoclave notation was not dated, but appeared. under the 06/23/1997 entry). On 06/28/1997, completed the ninth fermentation production run, which resulted in 7.3xlo12 total spores or 125ml. of 5.8xlolo spores/ml. During dilutions titers of raw spores two. (2) colonies of Bacillus globigii were noted on a single plate of nearly confluent Bacillus anthracis. During dilutions both contamination was noted. On 07/01/1997, aborted the fermentation run that began on 06/28/1997 due to Bacillus globigii contamination. According to notebook this batch was autoclaved on 07/01/1997. On 07/11/1997, completed the eleventh fermentation production run, which resulted in a reported. 5.34x1012 total spores or 175ml of 3.6x1010 spores/ml. 6.3xlo2 total spores. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 07/17/1997, completed the twelfth fermentation production run, which resulted in reported 5.2x1o12 total spores or 325ml of 1.7xlol spores/ml. Calculations using 325mls at 1.7xl010 spores/ml are actually 5.5x1012 total spores. Bacillus globigii contamination was noted. On 07/21/1997, lcompleted the thirteenth fermentation production run, which resulted in 25oml of 2.0xlo10 spores/ml (approximately 5.0xlo total spores). During dilutions after heat shock procedures one (1) colony of Bacillus globigii contamination was noted on one (1) dilution plate. On 07/29/1997, aborted the fermentation run that began on 07/24/1997 due to Bacillus globigii contamination. According to notebook this batch was autoclaved (contamination and autoclave notation was not dated, but appeared under the 07/29/1997 entry). 0505a.ec 3