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Working Copy Page 2 non-anthracis Bacillus cultures.. Bacillus subtilis is a non-pathogenic bacterium found ubiquitously in the environment. However, genomic DNA sequencing of the specific isolate of Bacillus subtilis discovered within the Post and Brokaw anthrax powders reveals. that it is genetically distinct from other known isolates of. Bacillus subtilis. Analyses of the Bacillus subtilis from both the Post and the Brokaw envelopes revealed that these two isolates are identical.. UsAMRIID is a military research institute located on the Fort Detrick, U.S. Army Post in Frederick, Maryland. Prior to the anthrax attacks in the Fall of 2ool, UsAmRIiD was. sawy ay? passassod yptem sattoaeaoqet *san (9t) uaaaxes Jo auo strain of Bacillus anthracis. Phenotypic and genetic analyses of more than 1,0oo Ames samples from the sixteen (16) Bacillus anthracis used in the anthrax letter attacks was derived from a sample known as RmR-l029. At the time of its concentrated, Bacillus anthracis Ames spores, assembled for the purpose of conducting numerous anthrax aerosol challenges b2 at USAMRIID. RMR-1029 was stored in the containment b7F suite within Building. of usAMRIID under the custody and control of Dr. Bruce Edwards Ivins. The Bacillus subtilis contaminant is not detected in RMR-1029 by phenotypic or genetic analyses. It is not known. and Brokaw spore powders. Since the contaminant is not found. in RmR-l029 and not found in the spore material mailed to Senators Leahy and Daschle, it is reasonable to conclude that. the contaminant was introduced during the growth process of the evidentiary material. Taken together, the presence of the Bacillus subtilis contaminant, and the phenotypic differences of the evidentiary spore powders, it is presumed that, on two separate occasions, a sample of RMR-1029 was used to grow spores, dried to a powder, packaged in an envelope with a threat letter, and mailed to the victims.. In November of 2006, upon consent provided by. UsAmRIiD Command, strains of Bacillus subtilis, were collected from Dr. Ivins's stock collection. All of the collected samples compared negatively to the Bacillus subtilis. contaminant isolated from the Post and Brokaw envelopes. In June of 2007, upon consent provided by UsAmRIID Command, environmental samples were collected from. space and equipment; but, ultimately compared negatively to the evidentiary Bacillus subtilis contaminant.. On November 1-2, 2007, the Washington Field Office. (WFo) AMERITHRAx Squads, with assistance from the Hazardous Materials Response Unit (Hmru), the Evidence Response Team (ERT), the Hazardous Materials Response Team (HMRT), and the Computer Analysis Response Team (cArt) conducted searches of