◆ SpookStack

FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID b6 03/28/2003 3 Continuation ofFD-302 of ,Page b7C received stock of B.a. Ames from BRuce IVINS.. Ivins went back to the original B.a. Ames slant and generated a new ae aeoy aen on aaoen ee ueboeeed aen daeg oo Japeo ut yeeed possible. Ivins provided his Ames strain to in the following manner: He retrieved his stock from the freezer, took out a loop and plated it on agar. He then gave the plate to In addition to the B.a. Ames stock, recalled obtaining an spore preparation from IvINS This material was generated from Ivins' stock Of Ames. To maintain uniformity of lineage among the laboratory groups workinq together, everyone already had their own Ames stocks. Investigators Division used Ivins' Ames stock.. was not sure of the origin. of the Ames used by. derived from the original. slant. various B.a. strains but not Ames. does not store much in the way of 'true' Ames stocks or spore preparations. It would be fairly obvious if vials were missing. However, someone could take a loop out without. notice. With the small volumes of Ames that Istores, if an. appreciable volume was gone, would notice. Istores numerous stocks of Ames variants. These materials are visually. medusa-like colonies, when plated and is not hemolytic. with aging,. the culture becomes grainy and blebs appear. Ames mutants appear yellow in color and display hemolytic activity. If a person did not know better, he might mistake the mutant material as not anthrax. has never had a reason to generate a large amount of B.a. Ames spores.. 9q b7C b7F