◆ SpookStack

FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID b6 b7C Continuation of FD-302 of ,On 04/17/2003 ,Page _ Each cryovial of primary stock in the archive contained approximately 0.5 mL of material. A secondary stock of a given B.a. most of the vials in the archive were never used in this manner. To generate a secondary stock of a particular strain, a loop of. approximately 10 microliters (uL) was pulled from a primary stock and cultured on either aqar or broth media.. never worked with the original Ames slants and. was not aware of how the Ames original slants were received by USAMRIID. never saw any of the original Ames materials and was not sure if the primary stocks in the archive were derived directly. from the original slant. had no knowledge of the B.a. Ames stocks generated by BRucE IvINs and believed that Ivins was gone by the time that. Jarrived at. UsAmRIId and thought that he gave his B.a. Ames stocks to described in detail the procedures for the shipping. of pathogenic samples. The guidelines differed for the transport of. virulent and avirulent organisms. For requests of virulent materials, such as B.a. Ames, regulations were followed, as defined. by the Select Agents Program of the CENTERS FOR DISEASE CONTROL AND PREvENTION (CDC). To receive Select Agents, the/requester had to be registered with the cdc. Prior to shipment of the pathogenic materials, transfer paperwork, or EAl01 documents, were processed through the appropriate channels.