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Ur 70 FD-302 (Rev. 10-6-95) ALL INFORMATION CONTAINED HEREIN IS UNCLASSIFIED DATE 12-16-2008 BY 60324 UC BAW/DK/TH FEDERAL BUREAU OF INVESTIGATION Date of transcription 09/10/2004 BRUCE E. IVINS, Principal Investigator, date of birth 04/24/1946, telephone number b6 was lntervlewed at b7C his place of employment,. "the United States Army Medical Research. Fort Detrick, Maryland.* After being advised of the identities of the interviewing agents and the nature of the interview, IvINs provided the following information:. In 1985, Ivins was concerned about the possibility of genetic variants of Bacillus anthracis (Ba) making their way into cultures which were grown by sweeping batches of spores off of a seed stock and plating these spores. Ivins noticed that when he took a full sweep of many spores to inoculate a plate. (sweep technique), he would get a more heterogeneous culture. In addition, when he passed a culture that was plated as b6 described above, each generation of passage would include more b7C aberrant colony morphologies. When Ivins used just one colony X more homogeneous culture with just a few aberrant colony dhgt Saaug? pto. ay? Aq pastape sem ay ybnoynty 'satbotoydaou that he should inoculate plates by. taking a sweep of spores from the seed stock, Ivins believed that growing spores in this way was contrary to pure microbiology culture technique. As a result, in 1985, IviNS paas mau e ugbaq o? quegs sauy t8eg neugbgeo ayg oaug padddp stock which he refers to as the "1985" sample. Ivins intended to use this sample as a seed stock so that he would not have to recall the sample name, number, or what was written on the tube containing the "1985" sample. He does not think that any of the their repository. Ivins cannot recall if he used just one spore from the "1981" sample to create his "1985" seed stock or if he used just a few "normal" spores. Ivins noted that there were. originally two "1981" original slants; however, there is now only one "1981" slant. Ivins does not know what happened to the second slant. The "1985" sample was being used as seed stock for intramuscular (IM) challenges, therefore UsAmRIID was not growing large batches of spores. Large batches of spores ar Investigation on 09/08/2004 at Frederick, Maryland 279A-WF-222936-USAMRIID - Q.35 File # Date dictated 09/10/2004 SA SA b6 by b7C This document contains neither recommendations nor conclusions of the FBl. It is the property of the FBI and is loan to your agency; it and its contents are not to be distributed outside your agency..