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FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID b6 b7C Continuation of FD-302 of ,On 03/09/2004 .,Page 2 the aerosol preparation laboratory where the nebulizers and all glass impingers (AgI) were prepared. Room. contained the aerosol hood line. No other rooms were used for aerosol work with. b6 Anthra Building noted that the door between b7C rooms and closed by screws put into place on b2 Locked the room side. said that in 1995, Ames work was b7F done in suite of Building at UsAMRIID while renovations were being done to Building recalled Ames aerosol challenges being conducted there during that period in suite The Bacillus anthracis (B.a Ames used in the challenges was grown in suite lof Building. at USAMRIID. The investigators would prepare b6 f solution is used per aerosol b7C The conical tubes were kept in the refrigerator in room. b2 challenge. of.Building noted that if any conical tubes b7F were missing from room someone would notice, however, no one would notice if liquid was taken out of the tubes. BRuce IvINS would place tape on top of the conical tubes, however, the tape could be easily replaced. No agents for the aerosol challenges ere kept in the walk-in cooler on the first floor of Building At the end of the aerosol challenge approximately six (6) or seven (7) ml of B.a. would remain. The B.a. that was not used from the hood. The remaining material, except for the Agis, that was used in the challenge would be placed into a bag and autoclaved Oo notaa yaeeeg yaer pareads aeer aeey aer Aep aye go pue aeg ae being removed from the hood line, and then taken to room for plating. After the plates had been left in the incubator overnight, they would be completely covered with growth. The plates were then read in the morning following a challenge. After the plates were read, they were placed into a bag and clearly uaboyaed ayn ugeaas aeem 'heq ayn ut sem uaboeaed qeym yhtm paggee was, and who the investigator was. The bags would remain in room until nearly overflowing, or until the number of bags in the room became an obstruction. The bags often sat in room for several days or weeks prior to being removed.. Inoted that aaaeed aya uo paaeadde yameeh yune moy yntm paaeuenaee dam after several days or weeks. The bags were then taken to the basement to be autoclaved. The bags were placed into metal garbage e aee aaeen aaen ue 'aaeeneane aee o? aeee 'aeaeeaeg aeg ut aee? leak in the bag. The animal caretakers at usAmRIID would autoclave.