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OO EE EE EEE eE © - To: Washington Field From: Washington wie Re: 279A-WF-222936-USAMRIID, 11/16/2004 system. The Cell Culture Group collects the penthouse water in large carboys (on an as needed basis), and utilizes the water for solid and liquid media preparations. CI staten that water purchased from Baxter Internationa ncorporated was used for “aerosol challenges and post-challenge plate dilutions during a the 2001 timeframes — This water-was pyrogen=free and contained no endotoxins. The aerosol challenge area gets deionized water from Building 1412's distribution system, with Millipore filtration units attached to several faucets for further purification. According a 0 the Cell Culture Group provided the steriliz sed in the aerosol challenges. Bruce Ivins would sometimes provide the —"--==sterile-water7—wht s* purchased “from-a7~commercial~source =" ~" . and stored in Suit in Building 1425. Purchased water was used, specifically, in the all glass impingers (AGI) in aerosol challenges. | ] ee are Information on the sou Water For USAMRIID. Fort Detrick operates it s own water treatment plant which purifies raw water from the Monocacy bé River. The treatment plant which utilizes sand filtration, b7c has not changed its method of treatment except for the discontinuation of adding fluoride starting in approximately 1996. [___]*is° provided annual reports of regulated contaminant monitoring results from 1996 to 2003. of usmerro's[_ | team stated that Building 1412 and 14 ave independent water purification systems. Building 1412 utilizes steam to distill water, and Building 1425 utilizes reverse osmosis (RO). Neither ‘building has had_w ades to the systems since its inception. (Since the interview, upgrades to the purification systems have been implemented in both Building 1412 and 1425.) - caod IG a Each laboratory has its own separate filtration units to provide an extra layer of purification. Many of these units are Millipore, Aqua Solutions, or Biocel brands. There are different models of each brand, but they all function si running the water through several layers of filters provided a list of all the water i ions its currently in the USAMRIID laboratories, and ia nash [provided the locations of each one.