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4 « To6:° Washington ricla@ron: Washington Field 6 Re: 279A-WF-222936, 01/11/2006 which Ivins calculated the amount of Ames spores which equaled a be certain amount of Ba Zimbabwe spores. . Notebook 4306, issued to Bruce Ivins on August 28, 2000 and entitled "Anthrax", contained experiments on various topics, including the comparison of Ba Vollum 1B on MicroDiagnostics Nutrient Agar versus Difco Nutrient Agar, the effect of temperature on spore counts of Vollum 1B, and whether Solid Agar medium was suitable for growing the Ba, strain V770-NPI-R. An experi ducted as a result of a conversation Ivins had with | who advised that the United States Department of Agriculture (USDA) freezes anthrax spores at -70 Celsius in 50% glycerol. Ivins wanted to determine whether 100%, 50%, and 25% glycerol solutions in water froze at -70 Celsius. Also contained in the notebook was an experiment to determine loss of counts due to transfer of spores from one tube to another. A was mentioned in this experiment. Another experiment involved spore counts on plates spread to dryness versus counts on plates not spread to dryness. Noteb i B i ie 8, 2000, with entries by was entitled a This notebook included spore-related studies on the ects of storage conditions on spore counts in suspension (on Difco tryptic soy agar), spore counts on different solid media (tryptic soy agar, nutrient broth agar, BHI agar, capsule agar, sheep blood agar, and chocolate agar) percent encapsulation of spores in preps (on capsule agar), pour plate versus spread plate comparisons (on nutrient agar; procedure written by BioPort), and percent of spores in preps that are refractile or non-refractile. Assisting with the BioPort study were It is unclear where ese individuals were employed. Notebook[___] was issued January 3, 1978 to Cent was entitled "Pathogenesis of Anthrax." yophilized an ampule of an unknown substance on np yophi lized a Ba Sterne sample. . Notebook Wi i 79 to | ee ee ce | mee CO ar “study involving a MDPH (now BioPort) antigen was d, which the antigen was adsorbed onto alh an eee then gave samples from the above top | and to for EF analysis. One page fronL___——d Notebook ws e res copied after a summary EC was written regarding th of [laboratory notebooks. Notebook was issued July