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FD-302a (Rev. 10-6-95) 279R-WF-222936 Continuation of FD-302 of oe ,On_01/03/06 _,Page__7 Since_approximately 1998 there was an old lyophilizer located in coon} USAMRIID_has recently replaced the old lyophilze: i new one. [ __}es never lyophilized Ba material. believes that somebody using a lyophilizer to dry the Ba material in the anthrax letters would have created a big mess, people would have gotten sick, and left a definite footprint. believes that acetone drying is a lot easier, safer, and does not require specialized equipment. An aerosol challenge usiyg dry Ba USAMRIID because of the political/fallout. [rout never store Ba in room technician would autoclave AGI and plate material before leaving laboratory. If the autoclave i: was already in use, would store material left to be autoclaved in the hood. Material waiting to be autoclaved could possibly be in the hood until the next day. ‘p: Y. A piece of autoclave indicator tape was affixed to material to be autoclaved. Usually the agent to be autoclaved would be written on the outside in case somebody had an exposure to the material. Writing the agent on the outside allowed for a more timely and appropriate post-exposure treatment of person’s exposed. Hot material, if not refrigerated or frozen, could only be in the hood or in the autoclave. [ [believes thawing frozen Ba is a “no, no”, since viability 0 @ sample is lost each time freeze-thawing occurs.