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To: Washington @ From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 b2 aerosol enges were i rooms[|__] and[ | in Building] eye and room in Building at the United ates Army Medical Research Institute of Infectious Diseases (USAMRIID) . Documentation collected shows that there were only 7 Primary Investigators (PI) involved i oted rified through_interview, were! ce Ivi bé and other personne category) documented as being involved in the aerosol process during this period as well. Sixteen of the 22 individuals documented were involved in ess ei ith B ae echn an D the 53 Ba aerosol challenges run,;__ sd ran 37 of them. Through interviews, it has been suggested thatL__ | ran most of the challenges because of the sensitivity of the challenges being run as part of the vaccine efficacy studies. The test animals utilized in the Ames aerosol challenges included rabbits, non-human primates (NHPs), and mice. The Ba used in the challenges was primarily produced and provided by Ivins. One batch of highly purified Ames spores produced for aerosol challenges was referred to as Reference Material Receipt t was a combined batch of spores produced by Ivins When this material began to run out, an extensive vaccine challenge was planned which required a very large amount of highly purified Ames spores. Ivins contracted Dugway Proving Grounds to produce Ames spores in fermenters and provide them to USAMRIID for use in this study. The Dugway Ames was purified by Ivins and subsequently combined with multiple batches of Ames spores produced by Ivins Jat USAMRIID using a Leighton-Doi broth technique. The combined Ames spore preparation was referred to as RMR 1029. RMR 1029 and 1030 were the primary sources of challenge material during the period for which Ba-challenge information was compiled. However, some challenges were conducted using other strains of Ba. The source of the Ames material used in each challenge is provided if it was documented or could be verified through interviews.