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To: Washington m4 From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 Information obtained from interviews indicated that standard protocol is and was for the post-challenge plates to be autoclaved in the challenge labs prior to disposal in the basement, meaning that this material should have been autoclaved b2 twice. Several technicians stated that this was a ee the byF practice. All material on the hot side of Building was considered to be "hot", and it was the opinion of several technicians that the PIs and their staff were not concerned if material was autoclaved before leaving a room or lab for disposal. The main concern was that material was autoclaved in the basement before leaving the hot side of the building. DOCUMENTED Ba AEROSOL CHALLENGES: The information below was taken from four aerosol exposure log books obtained from the Aerobiology Division, information furnished by Ivins regarding Ames stock distribution within USAMRIID, interviews of USAMRIID staff, and protocol proposals. Copies of the aerosol exposure log books are maintained in the 1A section of the captioned file. 12/9/1996 Exposure _97-007H, Protocol F96-17 PI: ech: Td) Room ood 4, animals exposed 12/11/1996 Exposure _97-008H =17 PI: Tech: Room Hood 4, 23 Rabbits exposed 12/12/1996 "Expt 7-009 ol F96-17 PI: Tech co Roo Hood 4, 22 Rabbits exposed 12/13/1996 Exposure 97-010 F96-17 PI: Tech: Room Hood 4, 21 Rabbits exposed 12/17/1996 Exp 7-011 1 F96-16 PI: Tech: Room Hood 4, 13 NHPs exposed