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Tor Washington i From: Washington Field : Re: 279A-WF-222936, 03/08/2005 bé bic Purification of Bacillus anthracis Ames Spores for Aerosol Challenge y "Preparation of Bacillus anthracis Spores for Aerosolization"; an April 1997 SOP prepared by Ivins entitled "Quantitation of Bacillus anthracis Colony Forming Units"; an April 1997 SOP prepared by Ivins entitled "Preparation of Bacillus anthracis Spores for Testing Aerosolization Efficiency"; and a March 1997 SOP prepared by Ivins entitled "Preparation of Bacillus anthracis Spores for Aerosol LD50 Determination". In the 2 a Ba Ames Primary Subculture was shipped to Battelle in April 2004. Ba Ames ‘"ready-to-spray" spores were shipped to Battelle between April and September 2004. Furthermore Po] Ivins visited Battelle for observation and advice between May and September 2004. Notebook #16, also labeled #3464, contained an entry by i July 27, 1994. Ivins reported that [ist a performing work with Ames spores in rabbits. Ivins also attached an August 17, 1994 graph showing a gamma- irradiation kil vi was px b - On_or about November 22, 1994, an experiment was _to_ be conducted to Ba Ames was one é strains used in this experiment.