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FD-302a (Rev. 10-6-95) e 279A-WF-222936-USAMRIID, 279A-BA-C101392 Continuation of FD-302 of L—idCQL On 06/06/2003 Page bleach. Swipes of biosafety cabinets following decontamination yielded no growth on culture media, but detectable levels of pxo1 and pxO2 in some samples. A study was conducted whereby pre- decontamination sampling was used to identify the 'hottest', or most contaminated, areas in a biosafety cabinet. Detectable levels of DNA were obtained from these areas after decontamination with bleach. The following USAMRIID personnel may provide further insights into the impact of various decontamination methods on s i ifi H UCE IVIN: and Internet searches may yield additional information related to decontamination of biological materials, including diagrams of key mechanisms of inactivation. C7 provided the Agents with a manuscript containing an overview of decontamination technologies and descriptions of the mechanisms of action of numerous decontaminants on micro isms. The docutent i = ored byl ft and a APPLICATIONS INTERNATIONAL CORPORATION (SAIC), NATIONAL CANCER INSTITUTE FREDERICK, FORT DETRICK, MARYLAND, 21702, telephone The manuscript is maintained in’ a IA envelope (FD- ~ 340) lso recommended the following textbook as a useful reference: DISINFECTION, STERILIZATION, AND PRESERVATION, 5th edition, Seymour S. Block, Editor, Lippincott Williams & Wilkins, Philadelphia, Pennsylvania, 2001. Photocopies of the first two pages of the textbook were provided to the Agents and are maintained in a 1A envelope. The Agents were also provided with copies of USAMRIID'S records of transfers of Bacillus anthracis that occurred from 1997 thr: quested in a letter dated June 5, 2003, from SSA The EA101 forms provided by USAMRIID are maintained in a 1A envelope.