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FD-302a (Rev. 10-6-95) 279R-WF-222936-USAMRIID be b7c the aerosol preparation laboratory where the_nebulizers and all glass impingers (AGI) were prepared. Room contained the aerosol hood line. No other rooms were used for aerosol work with »é Anthr. in Building! noted that the door between 7° rooms aL] wy b the room| side. closed by screws put into place on >= said that in 1995, Ames work was bye done in suite[__] of Buildin at USAMRIID while renovations were being done to Building recalled Ames aerosol challenges being conducted there during that period in suite Ames used in the challenges was grown in suite at USAMRIID. Th : : be per aeroso pe enge. kept in the refrigerator in room DIF of Building noted that if any conical tubes were missing from room someone would notice, however, no one would notice if liquid was taken out of the tubes. BRUCE IVINS would place tape on top of the conical tubes, however, the tape could be easily replaced. No agents for the aerosol challenges peers in the walk-in cooler on the first floor of Building At the end of the aerosol challenge approximately six (6) or seven (7) ml of B.a. would remain. The B.a. that was not used in the challenges was normally autoclaved prior to being removed from the hood. The remaining material, except for the AGIs, that was used in the challenge would be placed into a bag and autoclaved at the end of the day. The AGIs were sprayed with bleac jor to being removed from the hood line, and then taken to room for plating. After the plates had been left in the incubator overnight, they would be completely covered with growth. The pilates were then read in the morning following a challenge. After the plates were read, they were placed into a bag and clearly marked with what pathogen was in the bag, what strain the pathogen was, and who the investigator was. The bags would remain in room Cfuntii nearly overflowing, or until the number of bags_in the room became an obstruction. The bags often sat_in room several _ days or weeks prior to being removed. thatL__lwas fascinated with how much growth appeare € plates after several days or weeks. The bags were then taken to the basement to be autoclaved. The bags were placed into metal garbage cans in the basement, next to the autoclave, in case there was a leak in the bag. The animal caretakers at USAMRIID would autoclave